ABSTRACT
Abstract Despite decades of research, wound healing remains a significant public health problem. This study aimed to develop and evaluate a topical sodium alginate gel containing vancomycin (Van) loaded MMT NPs for wound healing applications. Van was loaded in MMT at different conditions (pHs of 6, 7 and temperatures of 40, 50 °C) (Van/MMT NPs). The optimum formulation (with the smallest particle size and a high value of zeta potential; 270.8 ± 77.35 nm and -35.96 ± 2.73, respectively) showed a high drug-loading capacity (entrapment efficacy of 96%) and a sustained release pattern of Van (95%) over 480 min. The optimum Van/MMT NPs were embedded into the sodium alginate (SA) gel (Van/MMT NPs/SA gel). The Van/ MMT NPs/SA gel showed a sustained and slow release pattern of Van (95%) over 50 h. FTIR tests revealed the electrostatic interaction between MMT and Van. The broth macrodilution tube method was used to determine the minimum inhibitory concentration (MIC) of Van, Van/ MMT NPs, and Van/MMT NPs/SA gel against Staphylococcus aureus. The results showed the promising antibacterial activity of Van/MMT NPs/SA gel, thus, this gel can be a promising formulation for the management of infected wounds
Subject(s)
Wound Healing/drug effects , Wound Infection/pathology , Bentonite/antagonists & inhibitors , In Vitro Techniques/methods , Vancomycin/agonists , Alginates/analysis , Wounds and Injuries/drug therapy , Pharmaceutical Preparations/administration & dosage , Spectroscopy, Fourier Transform Infrared/methods , Anti-Bacterial Agents/classificationABSTRACT
PURPOSE: To investigate amniotic membrane as a biological dressing in infected wound healing in rabbits. METHODS: The use of preserved amniotic membranes (AMs) was examined using 15 rabbits with experimentally induced wound infections on their backs. Healing was histologically evaluated during different phases including inflammation, granulation, epithelialization, and fibroplasia. The animals were distributed into three groups for histological study at seven, 14, 21, and 28 days post-wound induction. Group A did not receive treatment: the wound was left exposed and dry; Group B received a daily exposure treatment with collagenase; and Group C received one AM, which also remained exposed. RESULTS: A marked reduction of the inflammatory phase was observed in Group C at 21 days, and the granulation phase of this healing increased at 14 days. Epithelialization was similar among the three groups, and fibroplasia was more pronounced in Group C at 14 days. Furthermore, gradual collagen organization also began for the animals in Group B at 14 days. CONCLUSION: The amniotic membrane did not significantly alter the inflammation, epithelialization, or fibroplasia phases but did increase angiogenesis up to Day 14 compared with the dry dressing and collagenase treatments. .
Subject(s)
Animals , Male , Rabbits , Amnion , Biological Dressings , Wound Healing/physiology , Wound Infection/therapy , Collagenases/therapeutic use , Fibrosis , Granulation Tissue/physiopathology , Neovascularization, Physiologic , Random Allocation , Reproducibility of Results , Time Factors , Treatment Outcome , Wound Infection/pathologyABSTRACT
A twelve year-old Thai boy with fulminating Aeromonas hydrophila infection of the right arm following a snake bite was reported. He recovered uneventfully after appropriate antimicrobial therapy and surgical intervention.